p p70 s6 kinase  (Cell Signaling Technology Inc)

Journal: Cell Death & Disease

Article Title: Pellino1-mTOR/S6K1 signaling axis is a key pathogenesis for the development of polycystic kidney disease

doi: 10.1038/s41419-026-08479-6

Figure Lengend Snippet: a Immunoblot analyses were conducted for the indicated proteins in kidney tissues from rtTA and rtTA-Peli1 mice following 12 weeks of doxycycline administration. b Immunohistochemical analysis was carried out for p-S6 (Ser240/244) expression in kidneys at 12 weeks after doxycycline treatment in rtTA and rtTA-Peli1 mice. Scale bars, 50 μm. c Western blotting was used to measure Peli1 and S6K1 protein levels after infection with lentiviral particles containing Peli1 shRNA in HK-2 cells. d Pull-down assays were conducted on 293T cells co-expressing TAP-Peli1 and flag-S6K1; TAP-Peli1 was precipitated with S-beads, and S6K1 co-precipitation was detected by immunoblotting using an anti-S6K1 antibody. e Co-immunoprecipitation was performed for GFP-Peli1 and Flag-S6K1 co-expressed in HK-2 cells. GFP-Peli1 was immunoprecipitated with an anti-GFP antibody, and detection of co-precipitated Flag-S6K1 was done by immunoblotting using the same antibody.

Article Snippet: For immunohistochemistry, the sections were deparaffinized, treated with citrate buffer for antigen retrieval, stained with anti-P-S6 (Ser240/244) (Cell Signaling, #2215) and anti-Peli1 (Santa Cruz, #sc-271), and incubated using Vectastain Elite ABC kit (Vector Laboratories).

Techniques: Western Blot, Immunohistochemical staining, Expressing, Infection, shRNA, Immunoprecipitation